The distribution of nucleic acid chromophores at cellulose-water interfaces. 2. Thermodynamic parameters.

A Previous theoretical' model (O. L. Hörer, Adriana Grigorescu Rev. roum. Virol, 1992, 43, 33) allowed us to appraise the "absorbed" mole fraction chi of chromophores, using the Multiple Light Scattering Enhanced Absorbance (MLSEA) bichromatic photometry. In the present paper the data were used to express thermodynamic parameters, based on the Gibbs free enthalpy G. In terms of the ion exchange capacity of several chromatography papers used in the former paper, the RNA chromophore distribution at the interface was correlated with thermodynamic parameter, showing characteristic patterns. The thermally denatured DNA was studied in the same way and a new insight of the helix-coil transition in terms of ionic strength was achieved.