AI Article Synopsis
- Macrophages normally require both priming and triggering signals to secrete tumor necrosis factor (TNF)-alpha.
- Studies show that mice lacking gamma delta T cells produce less TNF-alpha and express lower levels of CD14 in response to lipopolysaccharide (LPS).
- When macrophages from these T cell receptor delta-/- mice are pre-incubated with gamma delta T cells, their ability to produce TNF-alpha is restored, indicating that gamma delta T cells help prime macrophages for activation through interferon-gamma (IFN-gamma) secretion.
Article Abstract
The secretion of tumor necrosis factor (TNF)-alpha from macrophages is regulated by both priming and triggering signals. We found that macrophages from mice lacking gamma delta T cells [T cell receptor (TCR) delta-/- mice], which lack the gene encoding the delta chain, produced only small amounts of TNF-alpha in response to lipopolysaccharide (LPS) and showed a reduced level of expression of CD14. Pre-incubation of macrophages from TCR delta-/- mice with gamma delta T cells from their TCR delta +/- littermates restored their capacity to produce TNF-alpha in response to LPS. The priming activity of gamma delta T cells was in part inhibited by neutralizing anti-interferon (IFN)-gamma monoclonal antibodies. Collectively, these results suggest that gamma delta T cells play a role in priming macrophages to a steady state of activation via IFN-gamma secretion, which allows them to produce TNF-alpha when exposed to LPS.
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| http://dx.doi.org/10.1002/eji.1830250551 | DOI Listing |
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