The vaccination of young piglets, with or without passive antibodies of colostral origin, was studied using the Thiverval strain. The various experiments, carried out under practical conditions, allowed us to observe the following phenomena:--in piglets without immunity of maternal origin we observed a classic primary reaction, with vigourous, lasting production of antibodies and resistance to the challenge;--in piglets with immunity of maternal origin, depending on when the vaccination was administered, the production of primary antibodies was non-existent or deficient; however, we noted that piglets vaccinated 35 days after birth resist the challenge.
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Microb Biotechnol
March 2023
Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - Center for Infection and Immunity of Lille, Lille, France.
Yarrowia lipolytica is a dimorphic oleaginous non-conventional yeast widely used as a powerful host for expressing heterologous proteins, as well as a promising source of engineered cell factories for various applications. This microorganism has a documented use in Feed and Food and a GRAS (generally recognized as safe) status. Moreover, in vivo studies demonstrated a beneficial effect of this yeast on animal health.
View Article and Find Full Text PDFMol Plant Microbe Interact
January 2023
ARVALIS Institut du Végétal, 91720 Boigneville, France.
Fungal genus causes diseases in a wide range of plants. Here, we report the first genome sequences of two strains of , the causal agent of the pasmo disease of flax (). The genome of the first strain, SE15195, was fully assembled in 16 chromosomes, while 35 unitigs were obtained for a second strain, SE14017.
View Article and Find Full Text PDFMicroorganisms
July 2022
UR BIOGER, INRAE, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
Increased drug efflux compromises the efficacy of a large panel of treatments in the clinic against cancer or bacterial, fungal, and viral diseases, and in agriculture due to the emergence of multidrug-resistant pathogenic fungi. Until recently, to demonstrate increased drug efflux, the use of labeled drugs or fluorescent dyes was necessary. With the increasing sensitivity of detection devices, direct assessment of drug efflux has become realistic.
View Article and Find Full Text PDFJ Extracell Vesicles
May 2022
Department of Biology, Indiana University, Bloomington, Indiana, USA.
Fungal phytopathogens secrete extracellular vesicles (EVs) associated with enzymes and phytotoxic metabolites. While these vesicles are thought to promote infection, defining the true contents and functions of fungal EVs, as well as suitable protein markers, is an ongoing process. To expand our understanding of fungal EVs and their possible roles during infection, we purified EVs from the hemibiotrophic phytopathogen Colletotrichum higginsianum, the causative agent of anthracnose disease in multiple plant species, including Arabidopsis thaliana.
View Article and Find Full Text PDFCytometry A
July 2022
AgroParisTech, INRAe, UMR SayFood, Université Paris-Saclay, Thiverval-Grignon, France.
Cultivability, viability, and vitality make it possible to characterize the behavior of a cellular population. Vitality was assessed using the kinetic parameters of specific metabolisms depending on whether the strains were used, for example, for the acidification of lactic acid bacteria or for CO production in fermenting yeasts. However, these methods are time-consuming.
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