Enteropathogenic Escherichia coli O111 bacteria very often produce a capsule-like polysaccharide exhibiting the same chemical composition as the O-specific chains of their lipopolysaccharide and being designated as O-antigen capsule. In this paper, a new simple procedure is described for the detection of this capsule polymer. O-antigen capsule polysaccharide and lipopolysaccharide of bacterial extracts were electrophoretically separated in agarose cetavlon gel, blotted onto nitrocellulose membranes and visualized by immunological methods. As a result of this procedure, the O-antigen capsule can be detected as a spot completely separated from the LPS (Figs. 1 and 2). Consequently, this method allows a clear discrimination of K+ and K- strains.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0934-8840(11)80564-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Two new enzymes that liberate undecaprenyl-phosphate to replenish the carrier lipid pool during envelope stress.
mBio
January 2025
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
The 55-carbon isoprenoid, undecaprenyl-phosphate (UndP), is a universal carrier lipid that ferries most glycans and glycopolymers across the cytoplasmic membrane in bacteria. In addition to peptidoglycan precursors, UndP transports O-antigen, capsule, wall teichoic acids, and sugar modifications. How this shared but limited lipid is distributed among competing pathways is just beginning to be elucidated.
View Article and Find Full Text PDFStructure and Biosynthetic Gene Cluster of Sulfated Capsular Polysaccharide from the Marine Bacterium sp. KMM 8419.
Int J Mol Sci
December 2024
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
sp. KMM 8419 (=CB1-14) is a Gram-negative bacterium isolated from a food-net mucus sample of marine polychaete collected in the Sea of Japan. Here, we report the structure and biosynthetic gene cluster of the capsular polysaccharide (CPS) from strain KMM 8419.
View Article and Find Full Text PDFA new subtype of serovar 6, K6b:O3, of Actinobacillus pleuropneumoniae based on genotypic analysis.
Vet Microbiol
November 2024
Nisseiken Co., Ltd., Tokyo, Japan; Nippon Institute for Biological Science, Tokyo, Japan.
We have analyzed the capsule (CPS) and the lipopolysaccharide O-Antigen (O-Ag) biosynthesis loci of fifteen field isolates of Actinobacillus pleuropneumoniae, including eleven North American and four Japanese ones, reactive to antisera against serovars 3, 6, 8 and/or 15. Ten North American isolates amplified a serovar 6-indicative fragment derived from the capsular loci, whereas one North American isolate and all four Japanese isolates amplified the serovar 6-indicative fragment as well as the serovar 3-indicative fragment. The five isolates producing a 3/6 banding pattern contain a type I CPS locus, named K6b, similar to serovar 6, but with differences in the cpxABCD and cpsABC gene sequences and the length of intergenic regions (modF-cpxA, and cpsC-cpsD).
View Article and Find Full Text PDFLipopolysaccharide as a Vaccine Target and the Role of Antibodies in Protection from Disease.
Vaccines (Basel)
October 2024
Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
is well recognized as a serious cause of infection in healthcare-associated settings and immunocompromised individuals; however, accumulating evidence from resource-limited nations documents an alarming rise in community-acquired . infections, manifesting as bacteremia and pneumonia as well as neonatal sepsis. The emergence of hypervirulent and antibiotic-resistant .
View Article and Find Full Text PDFSynergy between Group 2 capsules and lipopolysaccharide underpins serum resistance in extra-intestinal pathogenic .
Microbiology (Reading)
August 2024
Department of Clinical Microbiology, School of Medicine, Trinity College, Dublin, Republic of Ireland.
) is a major cause of urinary tract infections, bacteraemia, and sepsis. CFT073 is a prototypic, urosepsis isolate of sequence type (ST) 73. This laboratory, among others, has shown that strain CFT073 is resistant to serum, with capsule and other extracellular polysaccharides imparting resistance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!