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Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response.
J Immunol Methods
January 2025
ARUP Laboratories, University of Utah School of Medicine, Salt Lake City, UT, USA; Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. Electronic address:
Despite great advancements in the discovery of genetic variants underlying inborn errors of immunity, functional assessment of the immunological profile of patients in routine clinical practice remains challenging. The lymphocyte proliferation assay using H-thymidine incorporation has been the gold standard for decades for functional evaluation of T cells in the clinical laboratory, however, recently developed flow cytometric methods allowing for single cell analysis provide non-radioactive alternatives. Understanding the technical and analytical challenges of test development, validation and maintenance is essential for correct interpretation and test utilization, to assure appropriate and timely patient care.
View Article and Find Full Text PDFBiotin-Based Northern Blotting (BiNoB): A Cost-Efficient Alternative for Detection of Small RNAs.
Curr Protoc
December 2024
Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Advances in sequencing technology have led to the discovery of diverse types of regulatory RNAs. Differential transcript levels regulate cellular processes and influence disease severity. Identifying these variations through reliable methods is crucial for understanding the regulatory roles and disease mechanisms of regulatory RNAs.
View Article and Find Full Text PDFTumor specific delivery and radiation-enhanced tumor penetration of mesoporous silica nanoparticles for effective radionuclide therapy of ovarian peritoneal metastasis.
Int J Pharm
January 2025
Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT, USA. Electronic address:
Advanced ovarian cancer with peritoneal metastasis is challenging to treat. Limited tumor delivery and penetration of the therapeutics to deep tumor regions are significant barriers to effective treatment. The rising radiopharmaceuticals offer hopes for patients through targeted delivery.
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- Determining progesterone levels in mares' serum is crucial for identifying issues related to their estrus cycle or luteal insufficiency, but current methods lack quick, accurate results within two hours.
- This study assesses the effectiveness of the mini VIDAS device, a commercial enzyme-linked fluorescent assay, against the traditional radioimmunoassay (RIA) for progesterone measurement in mares.
- Results indicate a strong correlation between mini VIDAS and RIA measurements; however, the mini VIDAS may have limitations in precision at lower progesterone levels, which could affect diagnostic accuracy.
Revisiting sodium phosphotungstate and ammonium molybdate as nonradioactive negative-staining agents for single-particle analysis.
Acta Crystallogr F Struct Biol Commun
December 2024
University of Cologne, Institute of Biochemistry, Faculty of Mathematics and Natural Sciences, Zülpicher Strasse 47, 50674 Cologne, Germany.
This study reports the successful replacement of uranyl-based stains by either sodium phosphotungstate or ammonium molybdate in negative-staining electron microscopy. Using apoferritin as a test specimen, it is demonstrated that in combination with a facile on-grid fixation step, both stains yield comparable images to uranyl formate. Subsequently, using β-galactosidase, it is shown that both stains can also successfully be employed for single-particle analysis, yielding virtually indistinguishable results from uranyl formate.
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