The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, with the transcription startpoint being localized to 110-111 bp upstream of cpeB. The sequence of the promoter region bears no similarity to promoters reported for other cyanobacterial PE genes. Pentanucleotide repeats found upstream of some PE operons, particularly in the case of cyanobacterial strains capable of chromatic adaption, are not found in Synechococcus sp. WH7803; instead the sequence 5'-CGGTT-3' is repeated three times in the promoter region.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00023564 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chitin degradation by Synechococcus WH7803.
Sci Rep
November 2023
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
View Article and Find Full Text PDFProduction and cross-feeding of nitrite within populations.
mBio
August 2023
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
is an abundant photosynthetic bacterium in the open ocean, where nitrogen (N) often limits phytoplankton growth. In the low-light-adapted LLI clade of , nearly all cells can assimilate nitrite (NO), with a subset capable of assimilating nitrate (NO). LLI cells are maximally abundant near the primary NO maximum layer, an oceanographic feature that may, in part, be due to incomplete assimilatory NO reduction and subsequent NO release by phytoplankton.
View Article and Find Full Text PDFMolecular evidence of parallel evolution in a cyanophage.
PLoS One
February 2023
Department of Biological Sciences, University of Bergen, Bergen, Norway.
Antagonistic interactions between bacteriophage (phage) and its bacterial host drives the continual selection for resistance and counter-defence. To date, much remains unknown about the genomic evolution that occurs as part of the underlying mechanisms. Such is the case for the marine cyanobacteria Synechococcus and viruses (cyanophages) that infect them.
View Article and Find Full Text PDFIntegrated Proteomic and Metabolomic Analyses Show Differential Effects of Glucose Availability in Marine and .
Microbiol Spectr
February 2023
Departamento de Bioquímica y Biología Molecular-Campus de Excelencia Agroalimentaria CEIA3, Universidad de Córdoba, Cordoba, Spain.
Article Synopsis
- The study analyzed the effects of glucose (100 nM and 5 mM) on the proteome and metabolome of various marine cyanobacteria strains grown in different light conditions, revealing glucose metabolism through oxidative pentose and Calvin pathways without evidence of the Entner-Doudoroff pathway.
- It found variations in how glucose affected different strains, with some strains showing evidence of fermentation and indicating capabilities for metabolism even in darkness, suggesting a role for glucose in supporting survival.
- The research highlights that marine cyanobacteria are mixotrophic, utilizing both sunlight and organic matter, while also revealing a potential link between glucose assimilation and circadian rhythms through the proteins KaiB and KaiC.
Marine sp. Strain WH7803 Shows Specific Adaptative Responses to Assimilate Nanomolar Concentrations of Nitrate.
Microbiol Spectr
August 2022
Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario CeiA3, Universidad de Córdoba, Córdoba, Spain.
Marine , together with , contribute to a significant proportion of the primary production on Earth. The spatial distribution of these two groups of marine picocyanobacteria depends on different factors such as nutrient availability and temperature. Some ecotypes thrive in mesotrophic and moderately oligotrophic waters, where they exploit both oxidized and reduced forms of nitrogen.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!