AI Article Synopsis
- Three new pilin structural genes from Pseudomonas aeruginosa have been identified and their sequences published.
- By analyzing these new sequences alongside six existing ones, researchers categorized them into two distinct groups based on their DNA and protein structures.
- Epitope mapping showed that certain B-cell determinants, which can trigger an immune response, are primarily located at the end of the P. aeruginosa 1244 pilin, and many clinical isolates of P. aeruginosa possess these determinants on their pili.
Article Abstract
The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping immobilized peptides representing the pilin primary structure, with antipilin monoclonal antibodies revealed several B-cell determinants grouped near the carboxyl terminus of P. aeruginosa 1244 pilin. One determinant was found to reside near the pilin constant region. These determinants were found associated with the pili of 31 of 95 P. aeruginosa clinical isolates.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC186117 | PMC |
| http://dx.doi.org/10.1128/iai.62.2.371-376.1994 | DOI Listing |
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