Background: Substance P (SP) is increased in the inflamed intestine of Trichinella spiralis-infected rats, but the underlying mechanism is unknown. Interleukin 1 beta (IL-1 beta) messenger RNA and protein is expressed in the longitudinal muscle-myenteric plexus (LM-MP) of this model. Thus, the purpose of the study was to examine the ability of human recombinant IL-1 beta (hrIL-1 beta) to increase SP in LM-MP preparations from the intestine of noninfected rats.
Methods: LM-MP preparations were incubated with hrIL-1 beta, and immunoreactive SP (IR-SP) was assessed in the tissues by radioimmunoassay or immunohistochemistry.
Results: hrIL-1 beta increased IR-SP in the tissue in a time- and concentration-dependent manner, being maximal after 6 hours at a concentration of 10 ng/mL. The IR-SP could be depleted by scorpion venom, and immunohistochemistry revealed increased staining for SP within nerves of the LM-MP. The action of IL-1 beta was dependent on protein synthesis, was receptor mediated, and was not due to endotoxin contamination of the cytokine preparation.
Conclusions: hrIL-1 beta stimulates the synthesis of SP in myenteric nerves of rat intestine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0016-5085(93)91073-q | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Study on the course of Cryptosporidium baileyi infection in chickens treated with interleukin-1 or indomethacin.
Acta Vet Hung
June 1999
Department of Parasitology and Zoology, University of Veterinary Science, Budapest, Hungary.
The effects exerted by human recombinant interleukin-1 beta (hrIL-1 beta) and the prostaglandin inhibitor indomethacin on the course of Cryptosporidium baileyi infection in chickens were studied. Daily oocyst shedding was monitored by a quantitative method throughout the experiment. Humoral immune response to C.
View Article and Find Full Text PDFNaloxone differentially alters fevers induced by cytokines.
Neurochem Int
July 1997
Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA.
Interferon-alpha (IFN-alpha), a cytokine acting as an endogenous pyrogen and a putative activator of the opioid system, binds to opiate receptors in vitro. The mu opioid receptor antagonist, naloxone hydrochloride (NLX), attenuates IFN-alpha-induced increases in the firing rate of cold-sensitive neurons within thermosensitive areas of the brain. The influence of NLX on fevers induced by central endogenous pyrogens was investigated in rats.
View Article and Find Full Text PDFLack of evidence for an increase in interleukin-6 expression in adult murine bone, bone marrow, and marrow stromal cell cultures after ovariectomy.
J Bone Miner Res
December 1996
Department of Veterans Affairs Medical Center, Newington, Connecticut, USA.
Interleukin-6 (IL-6) has been implicated as a mediator of postmenopausal bone loss. In vitro studies of bone and bone marrow cells have suggested that estrogen regulates bone turnover by controlling the production of IL-6, a potent stimulator of osteoclastogenesis and bone resorption. To investigate this hypothesis in an in vivo model, we examined the effect of ovariectomy or estrogen replacement on IL-6 mRNA and protein expression in adult mouse bone and bone marrow in vivo and in marrow stromal cell cultures.
View Article and Find Full Text PDFHuman recombinant interleukin-1 beta induces thromboxane A2 release in polymorphonuclear leukocytes, macrophages and platelets: effect of IL-1 receptor antagonist.
Mol Cell Biochem
June 1996
Immunology Division, Medical School, University of Thessaloniki, Greece.
Prostaglandins and thromboxanes (Txs) are produced by polymorphonuclears (PMNs) and macrophages (Mphis) in response to various stimuli. PMNs were separated from other human blood cells and Mphis were separated from rat peritoneal lavage. In this paper we show that human recombinant interleukin-1 (hrIL-1) can stimulate the release of thromboxane B2 (TxB2) by PMNs and Mphis.
View Article and Find Full Text PDFThe role of nitric oxide in proteoglycan turnover by bovine articular cartilage organ cultures.
J Immunol
February 1996
Ferguson Laboratory, Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, PA 15261, USA.
Monolayer cultures of articular chondrocytes synthesize large amounts of nitric oxide (NO) following exposure to IL-1. The latter has antianabolic and procatabolic activities on these cells, but little is known about the role, if any, of NO in the integrated metabolic pathways of the chondrocyte. In the present study, the role of endogenously produced NO in both the synthesis and degradation of proteoglycans was investigated for the first time.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!