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Pharmacy services and psychiatric patient satisfaction among community-based health insurance users in Ethiopia.
Front Med (Lausanne)
November 2024
Department of Clinical Pharmacy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Background: The development of community-based health insurance (CBHI) was driven by the need to provide economic protection for the poor against unexpected healthcare expenses. This can lead to increased patient satisfaction with their overall care. Maintaining high levels of client satisfaction with pharmacy services is crucial for effectively treating patients with psychiatric disorders.
View Article and Find Full Text PDFNorthern Blotting: Protocols for Radioactive and Nonradioactive Detection of RNA.
Methods Mol Biol
November 2024
ncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France.
Northern blotting is a common technique in RNA biology, allowing to detect and quantify RNAs of interest following separation by gel electrophoresis, transfer to a membrane, and hybridization of specific anti-complementary labelled probes. In this chapter, we describe our protocol for efficient RNA extraction from yeast, separation on agarose gel, and capillary transfer to a membrane. We provide two different methods for strand-specific detection of several types of RNAs using oligonucleotide probes, the first using radioactive P-labelled probes, the second based on nonradioactive digoxigenin-labelled probes.
View Article and Find Full Text PDFSustainability of health outcomes of patients with type-2 diabetes mellitus after completing 6 months of remote tele-monitoring: Two-year results from a randomised controlled trial (OPTIMUM).
J Telemed Telecare
October 2024
SingHealth Polyclinics, Singapore.
Introduction: Meta-analysis shows that home tele-monitoring (HTM) improves glycaemic control in patients with type-2 diabetes mellitus (T2DM) up to 12 months, but their health outcomes after HTM cessation remains unclear. This study aimed to determine the health outcomes of these patients 18 months after completing 6 months of HTM, compared to standard care.
Methods: Patients with T2DM were enrolled in an open-labelled randomised controlled trial, aged 26 to 65 years, and suboptimal glycaemic control (HbA1c = 7.
Dynamic choice HIV prevention with cabotegravir long-acting injectable in rural Uganda and Kenya: a randomised trial extension.
Lancet HIV
November 2024
Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, CA, USA. Electronic address:
Article Synopsis
- A study aimed to enhance HIV prevention by providing participants in Uganda and Kenya with options for daily pills or long-acting injectable medication, allowing them to switch methods based on personal preference.
- The research involved 1,534 individuals at risk for HIV who previously participated in trials, with 984 agreeing to continue in the extension phase of the study.
- Results showed that the intervention group experienced a higher mean coverage of biomedical prevention (69.7%) compared to those receiving standard care, suggesting a potential benefit in offering treatment choices.
Kinetic characterization of amino acid activation by aminoacyl-tRNA synthetases using radiolabelled γ-[P]ATP.
FEBS Open Bio
September 2024
Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Aminoacyl-tRNA synthetases (AARSs) are fundamental enzymes that pair amino acids and tRNAs for protein synthesis. Aminoacylation occurs in two discrete steps. The amino acid is first activated by ATP, leading to an aminoacyl-adenylate intermediate and pyrophosphate (PP) formation.
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