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Preferential use of the VHIII immunoglobulin gene family for the synthesis of rheumatoid factors.
Adv Exp Med Biol
January 1996
INSERM 291, Montpellier, France.
Expressed swine VH genes belong to a small VH gene family homologous to human VHIII.
J Immunol
December 1994
Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.
The sequences of 34 swine H chain V-regions expressed with either IgG, IgA, or IgM C regions in adult swine and newborn piglets are described and compared. Sixteen of these V-regions were cloned by using a specific leader primer whereas 18 were cloned by anchored PCR. According to the operational criterion of VH family classification, i.
View Article and Find Full Text PDFHuman monoclonal rheumatoid factor derived from rheumatoid synovial cells monospecific for rabbit IgG.
J Rheumatol
April 1993
Division of Rheumatology/Allergy, University of California, Davis 95616.
Rheumatoid factors (RF) in rheumatoid arthritis (RA) are polyclonal autoantibodies directed against antigenic epitopes located in the Fc portion of the IgG molecule. Hybridoma technology has overcome the difficulty of their polyclonality, so that monoclonal RF (mRF) can be examined for their individual binding specificities and genetics. We isolated a monoclonal IgM RF secreting hybridoma (designated H4) from the rheumatoid synovial cells (RSC) of a patient with RA.
View Article and Find Full Text PDFV region diversity in human anti-insulin antibodies. Preferential use of a VHIII gene subset.
J Immunol
February 1993
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-2681.
Data on structures used by human antibody repertoires are derived principally from lymphoid malignancies and from autoantibodies that often express VH genes from the developmentally regulated fetal repertoire. To determine whether human immune responses generated by exogenous Ag use a pool of VH genes distinct from the fetal repertoire, nucleotide and predicted amino acid sequences were determined for five anti-insulin B cell clones from a type I diabetic patient treated with human insulin. The data show that a set of VHIII genes is preferentially used by human anti-insulin B cells.
View Article and Find Full Text PDFClonal analysis of a human antibody response. II. Sequences of the VH genes of human IgM, IgG, and IgA to rabies virus reveal preferential utilization of VHIII segments and somatic hypermutation.
J Immunol
February 1993
Department of Pathology, New York University School of Medicine, New York 10016.
The construction of mAb-producing cell lines has been instrumental in dissecting the fine specificities and genetic makeup of murine antibodies to exogenous and self Ag. The analysis of the genetic composition of human antibody responses has been hampered by the difficulty in generating human mAb of predetermined class and specificity. Using B lymphocytes from three healthy subjects vaccinated with inactivated rabies virus vaccine, we generated nine human mAb binding to rabies virus and analyzed the genes encoding their VH regions.
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