Using [32P]-labeled hybridization probe of plasmid pGB725 enriched with poly-TG-pairs of nucleotides, chicken genome fingerprints were obtained. High genetic informativity of hybridization profiles and the possibility of polymorphic loci in the telomeric macrochromosomes' regions was revealed.
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Genetika
April 2001
All-Russia Research Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Sciences, St. Petersburg, Pushkin, 189620 Russia.
Chicken lines produced by divergent selection for the functional adrenal reserves showed significant between-line differences in the content of corticosterone and other hormones (thyroxin, progesterone), as well as in body weight, early maturation, and egg yield. DNA fingerprinting with the pGB725 probe revealed molecular changes in genomic DNA of the chicken lines subjected to plus and minus selection. The genetic distances between the original population and the selected chicken lines, which were estimated from the molecular hybridization patterns, reflected the history of breeding.
View Article and Find Full Text PDFUsing [32P]-labeled hybridization probe of plasmid pGB725 enriched with poly-TG-pairs of nucleotides, chicken genome fingerprints were obtained. High genetic informativity of hybridization profiles and the possibility of polymorphic loci in the telomeric macrochromosomes' regions was revealed.
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