AlgU is homologous to the extreme heat shock sigma factor sigma E from enteric bacteria. In this work, AlgU was overproduced and purified and its function investigated at the biochemical level. AlgU was shown to associate with RNA polymerase and direct transcription of a target promoter. AlgU also exhibited multiple isoforms detected by 2D gel analysis. Treatment with a Ser/Thr phosphatase shifted the distribution of isoforms towards the basic side on 2D gels, suggesting that posttranslational modifications of AlgU may involve phosphorylation. The underphosphorylated forms of AlgU copurified with RNA polymerase. It is possible that phosphorylation affects AlgU activity or its stability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.1995.2703 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulatory role of PA3299.1 small RNA in Pseudomonas aeruginosa biofilm formation via modulation of algU and mucA expression.
Biochem Biophys Res Commun
January 2025
Department of Microbiology, University of Kalyani, Kalyani, 741235, India. Electronic address:
Small RNAs (sRNAs) have emerged as key regulators of transcriptional factors and components within regulatory networks that govern bacterial biofilm formation. This study aimed to explore the regulatory role of the PA3299.1 sRNA in controlling biofilm formation in P.
View Article and Find Full Text PDFAzotobacter biodiversity in Egypt using microbiological, biochemical, and molecular-biology multidisciplinary approach.
Genetica
January 2025
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, Alexandria, Egypt.
The presence of Azotobacter bacteria in the soil plays an important role in increasing its fertility and enhancing plant health. Azotobacter diversity depends on several environmental factors, particularly soil texture, pH, and nutrient content. The current study investigated the diversity of Azotobacter in various soil samples collected from 10 different governorates along the river Nile valley and its delta, Northern Mediterranean shore, Sinai, and Upper Egypt regions.
View Article and Find Full Text PDFEnvironmental alkalization suppresses deployment of virulence strategies in pv. DC3000.
J Bacteriol
November 2024
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, USA.
Plant pathogenic bacteria encounter a drastic increase in apoplastic pH during the early stages of plant immunity. The effects of alkalization on pathogen-host interactions have not been comprehensively characterized. Here, we used a global transcriptomic approach to assess the impact of environmental alkalization on pv.
View Article and Find Full Text PDFand analyses of the role of the Prc protease in inducing mucoidy in .
J Bacteriol
October 2024
Department of Microbiology, NYU Grossman School of Medicine, New York, New York, USA.
Unlabelled: In , alginate biosynthesis gene expression is inhibited by the transmembrane anti-sigma factor MucA, which sequesters the AlgU sigma factor. Cell envelope stress initiates cleavage of the MucA periplasmic domain by site-1 protease AlgW, followed by further MucA degradation to release AlgU. However, after colonizing the lungs of people with cystic fibrosis, converts to a mucoid form that produces alginate constitutively.
View Article and Find Full Text PDFmucinous phenotypes and operon mutant characteristics obtained from inpatients with bronchiectasis and their correlation with acute aggravation.
Front Cell Infect Microbiol
August 2024
Department of Infectious Diseases, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Objective: Although the mechanism is unclear, (PA) infection directly affects the frequency of acute exacerbations in patients with bronchiectasis. The aims of this article are to analyze the genetic mutation characteristics of the operon in PA, isolated from hospitalized patients with bronchiectasis, and to explore independent risk factors for frequent acute exacerbations of bronchiectasis.
Methods: Based on the number of acute exacerbations that occurred in the past year, these patients with bronchiectasis were divided into those with frequent acute exacerbations (Group A) and those with non-frequent acute exacerbations (Group B).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!