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Characterization of BoLA class II and by PCR-RFLP, cloning, and sequencing reveals sequence diversity in crossbred cattle.
Anim Biotechnol
November 2023
Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
The BoLA class II and genes in crossbred cattle were studied using PCR-RFLP, cloning, and sequencing techniques. Seventy-two crossbred cattle () were used in the current study. III and I restriction enzymes digested exon 2-3, revealing seven (III-A-G) and three (I A-C) motifs, respectively.
View Article and Find Full Text PDFGenetic variation of ABCB1 (rs1128503, rs1045642) and CYP2E1 rs3813867 with the duration of tuberculosis therapy: a pilot study among tuberculosis patients in Indonesia.
BMC Res Notes
July 2021
Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Jatinangor, Bandung, Indonesia.
Objective: The risk of contracting tuberculosis (TB) and the efficacy of TB therapy are affected by several factors, including genetic variation among populations. In the Indonesian population, data on the genes involved in drug transport and metabolism of TB therapy are limited. The aim of this study was to identify the genetic profile of the ABCB1 gene (rs1128503 and rs1045642) and CYP2E1 gene (rs3813867) in Indonesians with TB.
View Article and Find Full Text PDFPCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan.
Braz J Biol
June 2021
Centre for Animal Sciences & Fisheries, University of Swat, Swat, Pakistan.
Plasmodium vivax is the most common human malaria parasite in Asian countries including Pakistan. Present study was designed to explore the genetic diversity of plasmodium vivax genotypes based on Pvmsp-3α and Pvmsp-3βgenes using allelic specific nested PCR and RFLP assays markers from field isolates in district Mardan, Pakistan. Blood samples of 200 P.
View Article and Find Full Text PDFAssociations between genetic variants of the gene and production traits in Saanen goats.
Arch Anim Breed
May 2019
Faculty of Agriculture, Department of Animal Science, Ege University, İzmir, Turkey.
This study was conducted to determine the polymorphisms of the gene and their relationships with milk yield and components, litter size, birth weight, and weaning weight in goats. For this purpose, a total of 108 Saanen goats from two different farms (Bornova and Manisa) were used as animal materials. Polymorphisms at the exon 6 and the 3 flanking region of the gene were determined by using PCR-RFLP with I and I restriction enzymes and DNA sequencing analyses.
View Article and Find Full Text PDFGenetic diversity of -casein () and lactoferrin () genes in the endangered Turkish donkey () populations.
Arch Anim Breed
February 2019
Namık Kemal University, Department of Agricultural Biotechnology, 59030 Tekirdağ, Turkey.
In this study, the -casein () and lactoferrin () genes which were found in association with milk production traits in different animal species were studied firstly in Turkish donkey populations. A total of 108 donkeys from different regions of Turkey were used in order to reveal the different genotypes of and genes by using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods. To determine the genetic polymorphism, we attempted to digest a fragment of 235 bp of the gene and a fragment of 751 bp of the gene using I, and II, I and I restriction enzymes, respectively.
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