A method for the production of Marburg virus in preparative amounts has been developed. It is based on sedimentation of the virus from blood plasma of infected guinea pigs by ultracentrifugation followed by purification in sucrose density gradient or gel chromatography on macroporous glass sorbents. The optimal terms of blood collection in infected animals were determined. Purified virus did not lose its biological activity. Concentrated virus preparations were studied by electron microscopy and polyacrylamide gel electrophoresis.
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