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Development of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting .
Front Microbiol
January 2025
College Food Science and Light Industry, Nanjing Tech University, Nanjing, China.
A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.
View Article and Find Full Text PDFPrevalence of common diarrheagenic enterobacteriaceae in Iran (2000-2023): a systematic review and meta-analysis.
BMC Gastroenterol
January 2025
Department of Biostatistics, Payame Noor University, Karaj, Iran.
Objective: Bacterial gastroenteritis is a significant public health concern, capable of causing severe infections. Among the various pathogens involved, those belonging to the Enterobacteriaceae family are the most frequently isolated and associated with gastrointestinal disorders. This study aimed to investigate the prevalence of common diarrheagenic Enterobacteriaceae in Iran over the past two decades, from 2000 to 2023.
View Article and Find Full Text PDFGastrointestinal infections are widespread and a major cause of global mortality, mainly affecting young children and the elderly. In Germany, each resident typically experiences one episode yearly. After COVID-19 pandemic, gastrointestinal infection rates have significantly increased again.
View Article and Find Full Text PDF[Urinary Salmonella Enteritidis Carriage in a Patient Living with HIV].
Mikrobiyol Bul
January 2025
Sağlık Bilimleri Üniversitesi Şişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Kliniği, İstanbul.
Tüm dünyada önemli bir halk sağlığı sorunu olan non-tifoidal Salmonella (NTS) sıklıkla gastrointestinal enfeksiyonlara neden olmakta ve taşıyıcılığa yol açabilmektedir. NTS'nin idrarda izolasyonu ve idrar taşıyıcılığı oldukça nadirdir ve predispozan faktörlerin varlığında sıklığı artmaktadır. Kinolon dirençli Salmonella spp.
View Article and Find Full Text PDFResearch Note: Gut instincts: Salmonella contamination based on monitoring systems in the broiler sector.
Poult Sci
January 2025
Flanders Research Institute for Agriculture, Fisheries and Food - Technology and Food Unit, Belgium. Electronic address:
Salmonellosis is the second most important zoonosis in Europe, with consumption of contaminated broiler meat and its derivative products as an important source of infection. One of the many measures to prevent Salmonella contamination of poultry meat at the slaughterhouses is logistic slaughter, this means that per day the contaminated flocks are slaughtered after the Salmonella free flocks. However, this principle relies on a monitoring system at farm level that is prone to false negatives.
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