AI Article Synopsis
- Host-parasite interactions during coccidial infection are complex and not well understood, prompting research on how host responses eliminate the parasite.
- Researchers used two-color immunofluorescent staining to analyze duodenal tissue samples from TK strain chickens post-infection with Eimeria acervulina at various time points.
- Findings showed that sporozoites primarily interacted with CD8+ lymphocytes and macrophages, highlighting the role of the immune response in the infection process, while meront development varied significantly depending on the type of oocyst and the timing of infection.
Article Abstract
Host-parasite interactions during coccidial infection are complex, and host responses leading to parasite elimination are not well understood. Interactions between host cells and parasites were investigated using two-color immunofluorescent staining of duodenal tissue infected with Eimeria acervulina. Tissues were removed from TK strain chickens 24, 48, and 72 h after primary infection with 2.5 x 10(7) normal or irradiated oocysts and also following secondary infection with 2.5 x 10(7) normal oocysts. The tissues were embedded in freezing compound, frozen, sectioned, and fixed in cold acetone. Sections were stained with monoclonal antibodies to identify CD4+, CD8+, Ig+, and Bu1a+ lymphocytes, as well as macrophages and parasites. At 24 h after all infections, sporozoites were seen primarily in CD8+ lymphocytes and macrophages. Many CD8+ lymphocytes were seen at all times in infected tissues and frequently appeared to be in contact with infected epithelial cells. Sporozoites were occasionally seen in CD4+ lymphocytes but none were noted in Ig+ or Bu1a+ lymphocytes. Meront development was extensive after primary infection with normal oocysts, sparse after primary infection with irradiated oocysts, and moderate after secondary infection.
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| http://dx.doi.org/10.3382/ps.0741117 | DOI Listing |
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