AI Article Synopsis
- Opioid receptors in the brain act as key interaction points for opiates, particularly focusing on kappa-opioid receptor (kappa-OR) mRNA expression in various cortical areas.
- Researchers used specific labeling techniques to identify kappa-OR mRNA, noting significant expression in certain neuron layers, especially in pyramidal and multiform neurons.
- The study's results on kappa-OR mRNA distribution align with previous findings on kappa-OR protein, suggesting that combined assessments of mRNA and protein can enhance understanding of kappa-OR roles in both normal and disease states.
Article Abstract
Opioid receptors (OR) provide primary interaction sites of the human brain with opiates. Presently kappa-OR mRNA expression was studied in different cortical areas (A4, A10, A17) by in situ hybridization using digoxigenin-labeled oligonucleotides and an alkaline phosphatase-mediated color reaction. kappa-OR mRNA was expressed mainly in layers II/III and V pyramidal and layer VI multiform neurons. A4 giant pyramidal and A17 giant stellate neurons stood out labeled. These findings fit in with our data on kappa-OR protein distribution. Combined cellular assessment of protein and mRNA will enable the study kappa-OR expression under physiological and pathological conditions.
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| http://dx.doi.org/10.1016/0304-3940(95)11797-z | DOI Listing |
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