Cytokines produced by different types of cells function by cell to cell interaction both in vitro and in vivo. Over thirty different molecules react in almost all physiological phenomena, such as in the fields of immunology, hematology, endocrinology, neurology, cardiology and nephrology. Previous observations indicate that the abnormal production of cytokines plays a part of pathogenesis of diseases. Therefore, it is necessary to know the amount of cytokines in the biological specimens for recognizing the pathogenic significance of cytokines. In this paper, we show the EIA-Kits of cytokines which are purchased. And we demonstrate the methods, advantages and disadvantages in the different enzyme immunoassays (EIA, DELFIA and Chemiluminescen-EIA) of interleukin 6 which we extensively investigated from basic to clinical experiments may be routinely used in the clinical examination in the near future.
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