AI Article Synopsis
- The Limulus amoebocyte lysate (LAL) test was assessed for its effectiveness in quickly identifying high levels of gram-negative bacteria in urine samples from adults.
- Initial results showed that the LAL test successfully detected 33 out of 36 samples with significant bacterial presence, but three false negatives were due to low urine pH, which could be corrected.
- The LAL test demonstrated rapid results, with most samples yielding positive readings within 10 minutes, proving to be a reliable and efficient method for diagnosing clinically significant gram-negative bacteriuria.
Article Abstract
The Limulus amoebocyte lysate (LAL) test was evaluated for rapid detection of gram-negative bacteriuria in an adult patient population. Time to gelation of a standard LAL preparation was used as a measure of significant (greater than 10(5) bacteria per ml) gram-negative bacteriuria, and the results of 190 LAL assays were compared with quantitative urine cultures. Initially, 33 of 36 urine specimens containing greater than 10(5) gram-negative bacteria per ml were detected by LAL assay. The three false-negative LAL tests were the result of urine pH levels below the pH minimum for LAL gelation; neutralization of these urine specimens resulted in positive LAL assays and 100% correlation with culture results. All 36 bacteriuric urine specimens were LAL positive within 15 min, with the majority of assays (86.1%) being positive after only 10 min of incubation at 37 degrees C. These data compared favorably with gelation times of 15 min when 1 X 10(5) to 2 X 10(5) gram-negative bacteria per ml were added to sterile urine. Two urine samples obtained from male patients with culture-proven gonococcal urethritis yielded positive LAL assays. The LAL assay was shown to correctly differentiate 96.2% of urine specimens as containing less than 10(5) or greater than 10(5) gram-negative bacteria per ml. The results of this study have shown that the LAL test can be used as a rapid, simple, and reliable screening procedure for the diagnosis of clinically significant gram-negative bacteriuria.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC273741 | PMC |
| http://dx.doi.org/10.1128/jcm.13.1.158-162.1981 | DOI Listing |
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