The interaction of human serum low density lipoprotein with a non-ionic detergent, Tween 80, was investigated by sedimentation velocity measurements and Sepharose CL-4B gel chromatography. The properties of Tween 80-extracted apoprotein (apo B) were studied by sedimentation velocity and sedimentation equilibrium measurements, gel chromatography, electron microscopy and chemical cross-linking. Tween 80, at low concentrations, increased the sedimentation coefficient of LDL in 3% NaCl to a plateau value. At the same time, the effective Stokes radius of LDL expressed as the partition coefficient in gel chromatography became larger. The effect was reversed in part after protease treatment. A further increase of Tween 80 concentration caused the all-or-none dissociation of the entire mass of apo B from LDL. The remaining lipid core of LDL was not destroyed by detergent at 25 degrees C, but it was at 35 degrees C. The Tween 80-extracted apo B was purified by gel chromatography and its molecular weight and partial specific volume were determined by sedimentation equilibrium analysis in H2O and D2O buffers. It formed a complex of 1,200,000 molecular weight with Tween 80, with f/fo = 2.2. The molecular weight of the protein part of the complex was estimated to be about 500,000, corresponding to the total apo B in an LDL molecule. This result was confirmed by chemical cross-linking of apo B in Tween 80. Under an electron microscope the apo B-Tween 80 complex appeared as a flexible string, a little less than 1,000 A in length and 50 to 60 A in width.
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http://dx.doi.org/10.1093/oxfordjournals.jbchem.a133103 | DOI Listing |
J Ayurveda Integr Med
January 2025
Center for Clinical Research and Education, The University of Trans-Disciplinary Health Sciences and Technology, Bangalore, India; Internal Medicine - Cardiology, University of Michigan, Ann Arbor, MI, USA. Electronic address:
Background: Obesity is a rising risk factor for various diseases including cardiovascular diseases and Cancer. The limitations of targeted obesity-treatment approaches employed in the clinic presently underscore the importance of developing integrative management strategies for identification of specific biomarkers of obesity.
Objectives: Given the specificity of exosome/extracellular vesicle (EV) biomarkers, we aimed here to identify the EV biomarkers of Ayurveda treatment - Lekhana Basti - for Obesity.
J Oleo Sci
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Faculty of Food and Agricultural Sciences, Fukushima University.
Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flame ionization detection (GC-FID). In this study, analytical conditions were optimized using free and esterified sterol standards.
View Article and Find Full Text PDFJ Pharm Biomed Anal
January 2025
Analytical Research and Development, Merck & Co., Inc., Rahway, NJ 07065, United States. Electronic address:
The dynamic landscape of mRNA technology highlights the need for innovative quality control (QC) strategies. In this study, we described an efficient one-step digestion approach for concurrent generation of 5'- and 3'-end fragments, enabling simultaneous mRNA capping and poly(A) tail analysis. Tailored 10-23-type DNAzymes, designed from 5'- and 3'-Untranslated Regions (UTRs), selectively cleaved mRNA to release both the 5'-Capped or uncapped short fragments and 3'-Poly(A) tail cleavage products.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Northwest University Chang An Hospital, Faculty of Life Sciences and Medicine, Northwest University, Xi'an, Shaanxi 710069, China; Department of Clinical Pharmaceutics, Chang An District Hospital, Xi'an, Shaanxi 710118, China. Electronic address:
Immobilizing the target protein on a solid surface with controlled orientation, high specificity, and maintained activity is a proven strategy to enhance the stability of the protein. In this study, we employed an ultra-high affinity protein pair consisting of a mutant of colicin E7 Dnase and its corresponding inhibitor, immunity protein 7(Im7), to develop an immobilized α-adrenoceptor (α-AR) column. Briefly, we expressed α-AR fused with CL7 as a tag at its C-terminus in Escherichia coli cells.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand.
Direct thrombin inhibitors (designated as EuRL-DTIs) were partially purified from ethanol extracts of Euphorbia resinifera O.Berg latex. The obtained EuRL-DTIs comprised four major compounds: two isomers of phenolic compounds (CHO) and two amide compounds (tentatively identified as CHNO and CHNO), as identified by liquid chromatography and electrospray ionisation quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS), attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and/or nuclear magnetic resonance (NMR) spectroscopy.
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