We propose a method allowing the automatic determination of equilibrium constants and binding site concentrations for an immune serum composed of a series of homogeneous antibody subpopulations. It is then possible to decompose the 'Scatchard plot' in its straight components corresponding to each antibody subpopulation.

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http://dx.doi.org/10.1016/0010-468x(80)90107-5DOI Listing

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