A rapid, sensitive and specific high performance liquid chromatographic method for the estimation of 5'-methylthioadenosine in biological samples has been developed. A double-step chromatographic on Dowex-50, H+ form, and Affi-Gel 601 were required before the chromatographic separation of sample on Partisil 10 SCX. The yield is quantitative and the procedure is simple and highly reproducible.
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