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Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain.
Sci Rep
January 2025
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Azurin, a bacterial blue-copper protein, has garnered significant attention as a potential anticancer drug in recent years. Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active.
View Article and Find Full Text PDFSEC-MX: an approach to systematically study the interplay between protein assembly states and phosphorylation.
Nat Commun
January 2025
Department of Biological Sciences, Columbia University, New York, NY, USA.
A protein's molecular interactions and post-translational modifications (PTMs), such as phosphorylation, can be co-dependent and reciprocally co-regulate each other. Although this interplay is central for many biological processes, a systematic method to simultaneously study assembly states and PTMs from the same sample is critically missing. Here, we introduce SEC-MX (Size Exclusion Chromatography fractions MultipleXed), a global quantitative method combining Size Exclusion Chromatography and PTM-enrichment for simultaneous characterization of PTMs and assembly states.
View Article and Find Full Text PDFRemoval of dissolved organic matter in road runoff with sludge-based filters from the drinking water treatment plant.
Water Sci Technol
January 2025
China Construction Fifth Engineering Division Co., Ltd, Changsha, Hunan 410004, China.
Road runoff underwent treatment using a filter filled with sludge from drinking water treatment plants to assess its capacity for removing dissolved organic matter (DOM). This evaluation utilized resin fractionation, gel permeation chromatography, three-dimensional excitation-emission matrix fluorescence spectroscopy, and UV-Visible spectroscopy. The filter demonstrated enhanced efficiency in removing dissolved organic carbon, achieving removal rates between 70 and 80%.
View Article and Find Full Text PDFAnalysis of Total, Free, and Esterified Sterols and Triterpene Alcohols in Edible Oils using a Gas Chromatography-Flame Ionization Detector.
J Oleo Sci
January 2025
Faculty of Food and Agricultural Sciences, Fukushima University.
Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flame ionization detection (GC-FID). In this study, analytical conditions were optimized using free and esterified sterol standards.
View Article and Find Full Text PDFDNAzyme approach for simultaneous mRNA cap and poly(A) tail length analysis: A one-step method to multiple quality attributes.
J Pharm Biomed Anal
January 2025
Analytical Research and Development, Merck & Co., Inc., Rahway, NJ 07065, United States. Electronic address:
The dynamic landscape of mRNA technology highlights the need for innovative quality control (QC) strategies. In this study, we described an efficient one-step digestion approach for concurrent generation of 5'- and 3'-end fragments, enabling simultaneous mRNA capping and poly(A) tail analysis. Tailored 10-23-type DNAzymes, designed from 5'- and 3'-Untranslated Regions (UTRs), selectively cleaved mRNA to release both the 5'-Capped or uncapped short fragments and 3'-Poly(A) tail cleavage products.
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