By 3 weeks after castration the majority of spermatozoa in the ligated cauda epididymidis were dead and decapitated and there was a 46% reduction in the total number of spermatozoa. By 5 weeks after castration > 99% of spermatozoa had disappeared. Electrophoresis of cauda epididymal plasma (CEP) on polyacrylamide gels revealed that after castration the protein composition of CEP changed from a pattern specific to the epididymis to one similar to blood serum. These changes in the luminal content could be prevented by administering testosterone. When spermatoza from the cauda epididymidis were diluted in CEP from normal rabbits they remained motile for several hours at room temperature. Dilution in CEP from 3- ro 5-week castrated animals stimulated vigorous forward motility but this declined to very low levels after 40 min. The evidence indicates that dead spermatozoa can be removed from the epididymis after castration by mechanisms not involving granuloma formation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1530/jrf.0.0600065 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regional modulation of toll-like receptor signaling pathway genes in acute epididymitis in mice.
Andrology
July 2024
Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil.
Background: Region-specific immune environments in the epididymis influence the immune responses to uropathogenic Escherichia coli (UPEC) infection, a relevant cause of epididymitis in men. Toll-like receptors (TLRs) are essential to orchestrate immune responses against bacterial infections. The epididymis displays region-specific inflammatory responses to bacterial-derived TLR agonists, such as lipopolysaccharide (LPS; TLR4 agonist) and lipoteichoic acid (LTA; TLR2/TLR6 agonist), suggesting that TLR-associated signaling pathways could influence the magnitude of inflammatory responses in epididymitis.
View Article and Find Full Text PDFChlormequat chloride induced activation of calmodulin mediated PI3K/AKT signaling pathway led to impaired sperm quality in pubertal mice.
Food Chem Toxicol
March 2024
Department of Toxicology, School of Public Health, Peking University, Beijing, 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing, 100191, PR China. Electronic address:
Chlormequat chloride (CCC), as a widely used plant growth regulator, can cause impaired sperm quality and decreased testosterone synthesis in pubertal rats, but the underlying mechanism remains unclear. The purpose of this study was to elucidate the toxicokinetics and tissue distribution of CCC, as well as the possible mechanism of CCC-induced impairment in sperm quality. The concentration of CCC reached its peak 1 h after a single dose (200 mg/kg·bw) administration in mice plasma, and a bimodal phenomenon appeared in the testes, liver, and epididymis.
View Article and Find Full Text PDFThe impact of retrieval method and breed on the motility and kinematic parameters of fresh and post-thaw ram epididymal spermatozoa.
Acta Vet Hung
January 2024
2Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Street 138, H-4032 Debrecen, Hungary.
This study was conducted to develop ideal post-mortem gamete retrieval and conservation methods to establish a Hungarian ex-situ in vitro gene bank. Pairs of testes from German Mutton Merino (n = 7) and Hungarian Black Racka (n = 7) rams were collected at a slaughterhouse, transported to the laboratory and stored overnight (4-5 °C) before processing. Post mortem ram epididymal spermatozoa (REPS) were obtained from the cauda epididymidis by slice or incision methods.
View Article and Find Full Text PDFThe impact of percutaneous epididymal sperm aspiration on sperm quality in mice.
Reprod Fertil
April 2023
Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria.
Abstract: In laboratory mice, sperm quality is usually assessed in spermatozoa collected from the cauda epididymidis of freshly sacrificed males. Percutaneous epididymal sperm aspiration (PESA) is a non-terminal alternative that would allow repeated sperm collection for sperm quality assessment in living males. To test whether PESA is a suitable method to assess sperm quality, we compared sperm traits between samples collected by PESA vs the commonly applied terminal cauda epididymidis dissection.
View Article and Find Full Text PDFFibrinogen-Related Protein, Fgl2, of Hamster Cauda Epididymal Fluid: Enzymatic Characterization, and Identification of Fgl2-Binding Proteins and Ligand of Defective Hamster Sperm Organelles.
Int J Biochem Physiol
September 2023
Department of Biology, Chemistry and Environmental Health Science, Benedict College, USA.
The luminal environment of the mammalian epididymidis performs a dual function; sperm maturation and maintaining sperm viability. We previously identified a secretory protein (260/280KDa oligomers) of hamster cauda epididymal principal cells that binds to nonviable sperm. The 260/280KDa oligomers are composed of 64kDa FGL2 (fibrinogen-like protein-2) and 33kDa FGL1) (fibrinogen-like protein-1).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!