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The authors examined 214 CSF samples, 187 of which had been obtained by lumbar and 27 by ventricular puncture. Within 10' from time of collection, each of the CSF samples were centrifuged in a Shandow cytocentrifuge for 15' at 1500 r.p.m. Slides were made and stained according to the May-Grunwald-Giemsa method. Of the 214 samples, 137 were from patients suffering various neurological disorders while 77 were from patients affected by primitive or metastatic verified CSN neoplasms.

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