Four serological assays for detection of antibody reactivity against surface antigens of benign and malignant cells have been evaluated. In all four assays, antibody attached to the surface of a target cell is detected by a rosette of indicator red blood cells. Of the four assays (antibody mixed hemadsorption assay, immune adherence assay, anti-C3 mixed hemadsorption assay and protein A assay), the anti-C3 mixed hemadsorption assay showed highest sensitivity. By using purified IgM and IgG fractions we could demonstrate that, except for the protein A assay, all assays show reactivity both with IgG and IgM. Protein A assay detected only the IgG fraction, with high sensitivity. The optimization of the four assays is described and the importance of using multiple assays is emphasized.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0022-1759(80)90182-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Blood filtering system for COVID-19 management: novel modality of the cytokine storm therapeutics.
Front Immunol
May 2023
ERNAM - Nanotechnology Research and Application Center, Erciyes University, Kayseri, Türkiye.
The newly emerged coronavirus (SARS-CoV-2) is virulent, contagious, and has rapidly gained many mutations, which makes it highly infectious and swiftly transmissible around the world. SARS-CoV-2 infects people of all ages and targets all body organs and their cellular compartments, starting from the respiratory system, where it shows many deleterious effects, to other tissues and organs. Systemic infection can lead to severe cases that require intensive intervention.
View Article and Find Full Text PDFcytotoxicity and virucidal efficacy of potassium hydrogen peroxymonosulfate compared to quaternary ammonium compound under various concentrations, exposure times and temperatures against African swine fever virus.
Vet World
November 2021
Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Background And Aim: The selection and proper application of disinfectants are crucial to the prevention of many diseases, so disinfectants must be evaluated before being used for the prevention of African swine fever (ASF). Three disinfectant products belonging to the group of potassium hydrogen peroxymonosulfates, product A and product B, and a quaternary ammonium compound called product C, were examined for host cell cytotoxicity and the efficacy of ASF virus inactivation. The study parameters included various concentrations, exposure times, temperatures, and degrees of cytotoxicity.
View Article and Find Full Text PDFRoles of conserved residues in the receptor binding sites of human parainfluenza virus type 3 HN protein.
Microb Pathog
September 2021
Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. Electronic address:
Human parainfluenza virus type 3 (hPIV-3) entry and intrahost spread through membrane fusion are initiated by two envelope glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F) protein. Binding of HN protein to the cellular receptor via its receptor-binding sites triggers conformational changes in the F protein leading to virus-cell fusion. However, little is known about the roles of individual amino acids that comprise the receptor-binding sites in the fusion process.
View Article and Find Full Text PDFRemoval of dabigatran using sorbent hemadsorption.
Int J Cardiol
October 2019
University of Pittsburgh Medical Center, Williamsport, PA, USA; The Heart Doctor, Manheim, PA 17545, USA. Electronic address:
Background: The Redual PCI trial has demonstrated the safety of dabigatran and ticagrelor or clopidogrel combination in preventing strokes in patients with atrial fibrillation. There was 15.4% risk of hemorrhage in the dabigatran/ticagrelor or clopidrogel arm, lower than that of triple therapy with warfarin, aspirin and ticagrelor or clopidogrel.
View Article and Find Full Text PDFTicagrelor Removal From Human Blood.
JACC Basic Transl Sci
April 2017
Cardiovascular and Metabolic Diseases, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Gothenburg, Mölndal, Sweden.
The authors devised an efficient method for ticagrelor removal from blood using sorbent hemadsorption. Ticagrelor removal was measured in 2 sets of in vitro experiments. The first set was a first-pass experiment using bovine serum albumin (BSA) solution pre-incubated with ticagrelor, whereas the second set, performed in a recirculating manner, used human blood mixed with ticagrelor.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!