AI Article Synopsis
- The study aims to identify dehydrogenase and diaphorase enzymes in spinal ganglia neurons of 12-day-old chick embryos using specific medium modifications.
- The medium for dehydrogenase includes components like sodium salt substrate and phosphate buffer, while diaphorase uses NAD variants and higher NaCl concentration.
- The researchers propose calculating enzyme activity based on the optical density of histochemical preparations over time, and they discuss how to relate these findings to biochemical data and cell metabolism differences.
Article Abstract
In order to reveal dehydrogenase and diaphorase in spinal ganglia neurons of 12-day-old chick embryos in cryostat sections, the following modifications of the medium were used: for dehydrogenase - sodium salt substrate 50 mM, NAD or NADPh 0.75 mM, nitro-BT 0.61 mM, phosphate buffer pH 7.2 15 mM, NaCl 50 mM, MgCL2 5 mM, for diaphorase - NAD-N2 or NADHh-N2 0.78-0.66 mM, NaCl 100 mM. To compare relative activity of the enzymes (optic density of histochemical preparations determined cytophotometrically) it is suggested to calculate the values obtained during proportional development of the staining regarding the time unit (hour). The possibility to compare the data obtained with the results of biochemical investigations is discussed, as well as an attempt is made to represent graphically metabolic peculiarities of various cell types.
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