The application of the ELISA technique to dry blood samples is described with special reference to some parameters: the correspondence between the "confetti" eluate and the serum dilution, and the preservation time of the dry blood samples. A correlation is made between the results obtained with the confetti and those obtained with the sera. The study shows that the dry blood samples are a valuable tool for a large scale diagnosis of human African trypnanosomiasis during sero-epidemiological mass investigations.
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Biomacromolecules
January 2025
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
As an abundant renewable natural material, starch has attracted unprecedented interest in the biomedical field. Carboxylated starch particles have been investigated for topical hemostasis, but the powder may not provide physical protection or support for wounds. Here, we prepared macroporous cryogel sponges of methacrylated carboxymethyl starch (CM-ST-MA) containing a covalent and a calcium ionic double network.
View Article and Find Full Text PDFNutrients
January 2025
Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
High-fat diet (HFD)-induced obesity represents a significant challenge to male reproductive health, affecting approximately 13% of the global adult population. This comprehensive review synthesizes current evidence regarding mulberry ( L.) fruit extract's therapeutic potential for HFD-induced male reproductive dysfunction.
View Article and Find Full Text PDFJ Clin Med
January 2025
Department of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan.
Rheumatoid arthritis (RA) is an immune-mediated disease characterized by polyarthritis that affects the small joints of the bilateral upper and lower extremities. RA shares several common clinical symptoms with Sjögren's syndrome (SS), another rheumatic disease caused by the lymphocytic infiltration of exocrine glands, with dry eye and dry mouth being the two most common symptoms. Anti-Ro/SS-A antibodies, a diagnostic biomarker of SS, are positive in patients with RA at a certain rate.
View Article and Find Full Text PDFAnimals (Basel)
January 2025
Department of Animal Nutrition, Faculty of Veterinary Medicine, Afyon Kocatepe University, ANS Campus, Afyonkarahisar 03200, Türkiye.
This research was carried out to determine the effects of potassium humate on the lactation performance and metabolic parameters of dairy cows during the transition period. Potassium humate was added to the concentrate feed at the following levels: (a) control (0%), (b) 0.5%, (c) 1%, (d) 1.
View Article and Find Full Text PDFChildren (Basel)
January 2025
Department of Clinical Pathology Dr. Soetomo Academic Hospital, Faculty of Medicine Universitas Airlangga, Surabaya 60132, Indonesia.
(1) Background: This study is designed to establish thyroid-stimulating hormone (TSH) reference intervals tailored to different neonatal age groups and Indonesian local populations. (2) Methods: Dried blood spot neonatal TSH values, from 1 January 2022 to 31 December 2023, were used to establish the neonatal TSH reference intervals partitioned by sex, gestational age, and ethnic group at different neonatal ages. (3) Results: A significant difference in the reference intervals value was observed in sex, gestational ages, and parental ethnicity groups in different neonatal age subgroups ( < 0.
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