Prostaglandin E1 and E2 in bovine semen: quantification by gas chromatography.

Prostaglandins PGE1 and PGE2 extracted from bovine semen, purified via silicic column chromatography were quantified by gas chromatography as their methoxime methyl ester trimethylsilyl ether derivatives. The PGE2 and PGE2 concentrations of 19 bovine semen samples ranged from 395 +/- 225 and 487 +/- 407 ng/ml, respectively. A constant 1:1 ratio between PGE1 and PGE2 was observed. There was not relationship between PGE and sperm motility, but high sperm counts were generally associated with decreased PGE levels. The direct precursors of PGE1 and PGE2, i.e. 20:3n6 and 20:4n6, occurred in low concentrations compared to other related unsaturated fatty acids, i.e. 18:2n6 and 22:5n6 of the n-6 family.