AI Article Synopsis
- The study examined how L-glutamine is metabolized in isolated dog kidney tubules at concentrations of 1 and 5 mM, finding that glucose production accounted for 40% and 25% of the glutamine removed, respectively.
- Roughly half of the glutamine at 1 mM and a third at 5 mM was unaccounted for, likely due to oxidation.
- The presence of fatty acids altered the results significantly when bovine serum albumin was added, highlighting the necessity to use albumin carefully in kidney tubule experiments.
Article Abstract
The metabolic fate of L-glutamine (1 and 5 mM) was studied in isolated dog kidney tubules. At 1 and 5 mM substrate concentration, and after 60 min of incubation, glucose represented 40% and 25% of the glutamine removed; the glutamine unaccounted for (which was probably completely oxidized) represented about one-half and one-third of glutamine removal at 1 and 5 mM glutamine concentrations, respectively. Due to contaminating fatty acids, addition of dialysed bovine serum albumin (fraction V) greatly altered the metabolic fate of glutamine. This observation emphasizes the need for using albumin with great caution for experiments with kidney tubules.
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