A total of 10 male and 10 female 9-week-old commercial Mycoplasma-free broiler chickens was used in this test. Five males and 5 females were artificially infected by aerosol with a broth culture of Mycoplasma synoviae (MS) F10-2AS. The other 5 males and 5 females were used as noninfected controls. At 3, 9, and 12 weeks postinfection all birds were bled, and the blood was pooled into 4 groups: infected male, noninfected male, infected female, and noninfected female. Hemagglutination-inhibition (HI) tests for MS and Mycoplasma gallisepticum (Mg) were run on a selected group of known positive serums using the 4 pools as red blood cell (RBC) sources. The geometric mean HI titers of the 4 groups showed no significant differences between male and female or MS-infected and noninfected chickens as a source of RCB's for the MS or Mg HI test. These results indicate that if procedures are correct, there is no danger in using RBC's from MS-infected birds.
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