Physical characteristics of simian adenovirus SA7 virion were studied. The buoyant density of SA7 virions was found to be 1.356 g/cm3. The percentage of DNA calculated from the buoyant density value and determined with phosphorus directly was 11.6% and 11.8%, respectively. The S020,w and D020,w were 740 S and 3.34 x 10(-8)/cm2, respectively. From the hydrodynamic parameters of the virion and its volume and specific partial volume as well as percentage of DNA and DNA molecular weight, the molecular weight of SA7 virion was calculated and found to be 185.5 Mdaltons.
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