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Transplantation of vascular endothelial growth factor 165‑transfected endothelial progenitor cells for the treatment of limb ischemia.
Mol Med Rep
October 2015
Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
The present study aimed to investigate the effects of neovascularization in rabbits with limb ischemia transplanted with vascular endothelial growth factor (VEGF)165‑transfected endothelial progenitor cells (EPC). Bone marrow mononuclear cells were isolated by gradient centrifugation, cultured in M199 culture medium and induced into EPCs using VEGF, basic fibroblast growth factor, and insulin‑like growth factor‑1, and subsequently identified. The EPCs were transfected with Adv‑green fluorescent protein‑VEGF165 and the proliferation potential of the cells was determined using an MTT assay.
View Article and Find Full Text PDFSelective catheterization and embolization of both profunda femoris arteries were performed on eight mongrel dogs. In four dogs, Gelfoam alone (one side), and Gelfoam soaked in Sotradecol (contralateral side) was the embolic agent. In the other four dogs, Avitene in saline (one side), and Avitene suspended in Sotradecol (contralateral side) was the embolic agent.
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