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Elemental composition of human airway surface fluid in healthy and diseased airways.
Am Rev Respir Dis
December 1993
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
The fluid that covers the surface of conducting airways (airway surface fluid, ASF) is a critical component of one of the first defense mechanisms of the lung against microbial and other environmental insults. Despite its physiologic importance, ASF is one of the only fluids in the human body whose composition remains poorly defined and understood. Attempts to analyze ASF have been hampered greatly by the fact that it exists only as a very thin layer covering the mucosal surface of airway epithelia.
View Article and Find Full Text PDF[Ultramicro-ELISA for the detection of IgM antibodies anti M. leprae].
Rev Inst Med Trop Sao Paulo
June 1993
Instituto de Medicina Tropical Pedro Kouri, Siboney, Ciudad Habana, Cuba.
The availability of an ultramicroanalytic system (SUMA) and species-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M.
View Article and Find Full Text PDFPrevention of allergic diseases and IgE dosification in cord sera.
Allergol Immunopathol (Madr)
May 1991
Juan Manuel Márquez Pediatric Hospital, Havana, Cuba.
In 1986, a Program on Prevention of Allergic Diseases was initiated in our country which was possible on account of the organization conditions of our health system. The program includes IgE measurement in umbilical cord serum by means of an ultramicroanalytic technique for IgE quantification. This is an heterogeneous sandwich-type immunoassay that requires only 10 microliters of reactive on each reaction step and its processing is carried out on the Cuban SUMA equipment.
View Article and Find Full Text PDFBichromatic absorbance measurements on RNA-impregnated paper disks may be applied to the ultramicroanalytic evaluation of the ion exchange capacity (IEC) of different chromatography papers. A newly defined absorbance parameter and the curves of its variation in terms of IEC are used to this purpose.
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