We examined the optimal conditions for maintaining normal human hematopoietic stem cells without cryopreservation. The effects of serum, HEPES, CPD and storage temperature were studied. Stem cell integrity (CFU-c, CFU-e, and BFU-e) was optimally maintained when the bone marrow cells were suspended in a medium containing 40% fetal calf serum, 25 mM HEPES, and 12.28% CPD and maintained at 4 degrees C. For example, under these storage conditions the recovery of functionally intact early erythroid stem cells, BFU-e, was 84% after 2 days, 44% after 4 days and 12% after 7 days. These results indicate that non-frozen short-term storage of human bone marrow may be of clinical utility in bone marrow transplantation.
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