Cells of Acanthamoeba castellanii release microexudates into the culture medium. The precursor material of the microexudates can be separated into four fractions on the basis of their solubility. One of these fractions carries a growth inhibitor, which is normally neutralized by other components of the precursor. A crude preparation of growth inhibitory material added to synthetic culture medium marks the growth of amoebae dependent on the quality of the substratum, and on the initial population density.
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