A novel method for the purification of the anti-T lectin from peanuts is described. The method is simple, easy to perform and requires no special reagents. The lectin is obtained in a highly purified from comparable to that prepared by affinity chromatography and large scale preparations are readily made. The lectin was shown by serological tests to have anti-T activity and is suitable for detection of minor T antigen activation on red cells.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.3109/08820138109093460 | DOI Listing |
Pak J Pharm Sci
July 2023
Thrombosis and Vascular Diseases Laboratory, School of Health and Biomedical Sciences, Royal Melbourne Institute of Technology (RMIT) University, Bundoora, VIC, Austra.
T-activation polyagglutination can be caused by bacteria or viruses and has been associated with haemolytic anaemia. Coronavirus disease-19 (COVID-19) is also associated with haemolytic anaemia. The presented study aims to determine T activation polyagglutination in critically ill COVID-19 patients.
View Article and Find Full Text PDFPediatr Blood Cancer
August 2021
Divisions of Laboratory Medicine and Hematology, Center for Cancer and Blood Disorders, Sheikh Zayed Campus for Advanced Children's Medicine, Children's National Hospital, District of Columbia, Washington.
Background: T-antigen activation usually occurs upon red blood cell (RBC) membrane cryptantigen exposure due to bacterial enzymes. Although uncommon, the condition is probably underrecognized. There is concern about hemolysis after plasma and plasma-containing platelet transfusions due to naturally occurring anti-T antibody in healthy blood donors.
View Article and Find Full Text PDFArq Bras Cardiol
February 2021
Universidade de São Paulo Faculdade de Medicina Hospital das Clínicas Instituto do Coração, São Paulo, SP- Brasil.
Background: The histopathological characteristics of Chagas disease (ChD) are: presence of myocarditis, destruction of heart fibers, and myocardial fibrosis. Galectin-3 (Gal-3) is a biomarker involved in the mechanism of fibrosis and inflammation that may be useful for risk stratification of individuals with ChD.
Objectives: We sought to evaluate whether high Gal-3 levels are associated with severe forms of Chagas cardiomyopathy (CC) and whether they are predictive of mortality.
Exp Parasitol
December 2019
Laboratory of Infectious Diseases of Domestic Animals, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, PE, Brazil.
The ability to differentiate from the proliferative (tachyzoite) to the latent (bradyzoite) stage of isolates of Toxoplasma gondii recombinant genotypes (I/II/III and I/III) and reference strains from a clonal line (RH and ME49) was investigated in this study. Two isolates from chicken (#114 and #277; ToxoDB) and 3 from pigs (#114; ToxoDB) were the subjects for evaluation. The isolates were grown in cell culture under 2 different conditions: culture medium at pH 7.
View Article and Find Full Text PDFPLoS Pathog
November 2018
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America.
Trypanosoma cruzi infection is characterized by chronic parasitism of non-lymphoid tissues and is rarely eliminated despite potent adaptive immune responses. This failure to cure has frequently been attributed to a loss or impairment of anti-T. cruzi T cell responses over time, analogous to the T cell dysfunction described for other persistent infections.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!