Fluorescence photobleaching recovery curves on a pair of membranes at various separations were calculated from a detailed knowledge of the variation in relative illumination areas and intensities as well as in relative contributions to the collected intensity with membrane separation. The observed diffusion coefficients were found to be relatively insensitive to membrane separation in all cases. Only for membranes with very different mobilities are there significant differences in fractional recoveries. Systematic variations in fractional recoveries with cell thickness may be indicative of differential mobility in the apical and basal membranes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jsscb.380170303 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Irradiation and DOM mediate lead release from polyvinyl chloride microplastics in natural surface water.
J Hazard Mater
January 2025
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address:
Polyvinyl chloride (PVC) is a widely used plastic, but the potential risk of heavy metal additive release from PVC microplastics (MPs) has not been fully explored. This study evaluates the release of lead (Pb) from recycled PVC MPs under natural conditions. The released Pb concentration in the dark was 1079.
View Article and Find Full Text PDFEngineering covalent small molecule-RNA complexes in living cells.
Nat Chem Biol
January 2025
University of Innsbruck, Institute of Organic Chemistry and Center for Molecular Biosciences (CMBI), Innsbruck, Austria.
Covalent labeling of RNA in living cells poses many challenges. Here we describe a structure-guided approach to engineer covalent RNA aptamer-ligand complexes. The key is to modify the cognate ligand with an electrophilic handle that allows it to react with a guanine at the RNA binding site.
View Article and Find Full Text PDFImmobile Integrin Signaling Transit and Relay Nodes Organize Mechanosignaling through Force-Dependent Phosphorylation in Focal Adhesions.
ACS Nano
January 2025
Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore.
Transmembrane signaling receptors, such as integrins, organize as nanoclusters that provide several advantages, including increasing avidity, sensitivity (increasing the signal-to-noise ratio), and robustness (signaling threshold) of the signal in contrast to signaling by single receptors. Furthermore, compared to large micron-sized clusters, nanoclusters offer the advantage of rapid turnover for the disassembly of the signal. However, whether nanoclusters function as signaling hubs remains poorly understood.
View Article and Find Full Text PDFApplication of biomass carbon dots in food packaging.
Environ Sci Pollut Res Int
January 2025
College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Since its discovery, carbon quantum dots (CDs) have been widely applied in cell imaging, drug delivery, biosensing, and photocatalysis due to their excellent water solubility, chemical stability, fluorescence stability biocompatibility, low toxicity, and preparation cost. However, the low fluorescence yield and poor surface structure limit the application of CDs. Heteroatom doping is considered an ideal method to improve CDs' optical and electrical properties.
View Article and Find Full Text PDFA stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging.
Nat Commun
January 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains challenging.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!