Spontaneous expulsion of a human ovum fecundated in vitro and transferred into the uterus occurred after 10 1/2 weeks of amenorrhoea. The oocyte had been collected after stimulation by human menopausal and chorionic gonadotrophins (hMG and hCG). The techniques of pre-ovulatory oocyte collection, fecundation, in vitro culture and of reimplantation of the embryo are briefly described.
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Narra J
December 2024
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Endometriosis remains a significant challenge for reproductive-aged women and is frequently associated with infertility. Although in vitro fertilization (IVF) is used to address infertility in women with endometriosis, its effectiveness in this context is still debated, particularly in developing countries such as Indonesia, where IVF remains a major challenge. The aim of this study was to investigate the success rates of early stages of IVF in women with and without endometriosis.
View Article and Find Full Text PDFAim: Within the in vitro fertilization (IVF) process, to evaluate the possibility of using the state of the meiotic spindle of oocytes as an indicator of maturity in order to optimize the timing of vitrification.
Patients And Methods: In the presented report, the cause of couple infertility was a combination of a 38-year-old female and 43-year-old male with azoospermia, which was an indication for oocyte vitrification. Oocyte polar bodies and optically birefringent meiotic spindles were visualized by polarized light microscopy and their states and relative positions were used as indicators of oocyte maturation, i.
Taiwan J Obstet Gynecol
January 2025
Reproductive Medicine Center, Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan; Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan. Electronic address:
Objective: To evaluate the effectiveness of oocyte retrieval using a single-lumen aspiration needle compared to a double-lumen aspiration needle in IVF (in vitro fertilization) patients.
Materials And Methods: This retrospective cohort study included all IVF cycles between 1st January, 2020 and 31st December, 2022 in Changhua Christian Hospital, Taiwan. Pairwise comparisons were conducted through propensity score matching analysis at a 1:3 ratio according to patients' age, anti-Müllerian hormone levels, and body weight.
Reprod Biol Endocrinol
January 2025
King's College London, London, UK.
Ovarian stimulation (OS) is a crucial component of clinical IVF treatment that strongly influences outcomes. As such, it is useful to understand the indicators for successful OS during IVF. As OS leads to multiple follicular recruitment, it can be quantified as number of oocytes retrieved.
View Article and Find Full Text PDFTheriogenology
December 2024
Department of Theriogenology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, 54000, Pakistan. Electronic address:
We hypothesized that human chorionic gonadotropic (hCG) could replace LH in the maturation media for buffalo oocytes, and hCG administration before ovum pick-up (OPU) enhances in-vitro development of buffalo oocytes. Objectives were 1) to investigate the effect of hCG supplementation on nuclear maturation, oocyte development, and granulosa cell mRNA abundance of genes related to growth and antioxidant pathways and 2) to determine the effect of hCG administration before OPU on in-vitro oocyte development. In Experiment 1, buffalo oocytes retrieved from slaughterhouse ovaries were maturated in the media supplemented with 0.
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