The effect of water and butylacetate contained in butanol used in production of potassium benzylpenicillin on the yield and quality of the latter was studied. It was found that normal butyl alcohol with the moisture level not more than 0.2 per cent provided the optimal conditions for the antibiotic production. Butyl acetate contained in butanol in an amount of 25 per cent was shown also to have practically no effect on the yield and quality of benzylpenicillin.

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