[Alpha-tocopherol and actinomycin D regulation of ubiquinone and protein biosynthesis in rat liver in E-hypovitaminosis].

In vitro experiments with the liver preparations from rats with E-hypovitaminosis revealed a more intensive incorporation of [14C]phenylalanine into ubiquinone induced by alpha-tocopherol. Actinomycin D inhibiting the DNA-dependent RNA synthesis decreases the incorporation intensity. A 30 min preincubation of the liver preparations with thesis. In case of preliminary addition of actinomycin D or its simultaneous introduction with alpha-tocopherol the latter has no stimulating effect on the intensity of [14C] phenylalanine incorporation into ubiquinone. When studying incorporation of [14C]phenylalanine into the total protein of the liver of rats with E-hypovitaminosis a tendency is revealed to an increase in the protein biosynthesis intensity in case of preincubation with alpha-tocopherol and to inhibition of the labelled precursor incorporation under conditions of preincubation with actinomycin D. The data obtained permit suggesting that the alpha-tocopherol effect on the ubiquinone biosynthesis may occur due to its effect on the RNA synthesis.