The capacity of human spermatozoa to penetrate zona-free hamster ova was used to investigate several methods of freezing and thawing sperm. No differences in postthaw sperm viability were obtained after cooling spermatozoa either with a complex egg yolk cryoprotective medium or glycerol. The in vitro fertilizing capacity of postthaw spermatozoa frozen with a moderate freezing rate was similar to that of spermatozoa frozen with a slow freezing rate. Significantly higher in vitro fertilization percentages were found when the spermatozoa were thawed at an environmental temperature of 22 degrees C (mean 51%) or 37 degrees C (mean 52%), as compared with spermatozoa thawed at 4 degrees C (mean 37%). Individual differences of in vitro fertilization percentages are discussed in relation to changes in motility. It is demonstrated that a high prefreeze in vitro fertilization percentage is no guarantee of a high postthaw fertilizing ability.

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0015-0282(16)45738-9DOI Listing

Publication Analysis

Top Keywords

vitro fertilization
12
vitro fertilizing
8
fertilizing capacity
8
human spermatozoa
8
freezing thawing
8
spermatozoa frozen
8
freezing rate
8
fertilization percentages
8
spermatozoa thawed
8
spermatozoa
7

Similar Publications

The objective of this study is to explore the impact of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) in female undergoing assisted reproductive technology (ART) on reproductive outcomes. A literature search was performed using electronic databases (PubMed, EMBASE, Web of Science, CNKI, Wanfang data, Geen Medical, and Cochrane Library). Risk ratio (RR), odds ratio (OR), and mean difference (MD) with 95% confidence intervals (CI) for various outcomes were presented.

View Article and Find Full Text PDF

In vitro fertilization (IVF) is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its competence. Here, we demonstrated that a nuclear coding gene, methyltransferase-like protein 7A (METTL7A), improves the developmental potential of bovine embryos.

View Article and Find Full Text PDF

Objective: We investigated whether the addition of a luteal phase support drug benefits pregnancy and perinatal outcomes in modified natural-cycle frozen-thawed embryo transfer (mNC-FET) for women up to the age of 35 years.

Methods: We analyzed the clinical data of 3658 mNC-FET cycles of women up to the age of 35 years from the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from January 2018 to December 2020 in a retrospective cohort study. The cycles were divided into three groups based on the luteal phase support protocol used.

View Article and Find Full Text PDF

Background: The diagnosis of depression or anxiety treated by SSRIs has become relatively common in women of childbearing age. However, the impact of gestational SSRI treatment on newborn thyroid function is lacking. We explored the impact of gestational SSRI treatment on newborn thyroid function as measured by the National Newborn Screening (NBS) Program and identified contributory factors.

View Article and Find Full Text PDF

Infertility has emerged as a significant global health concern. Assisted reproductive technology (ART) assists numerous infertile couples in conceiving, yet some experience repeated, unsuccessful cycles. This study aims to identify the pivotal clinical factors influencing the success of fresh embryo transfer of in vitro fertilization (IVF).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!