AI Article Synopsis
Article Abstract
Rat brain homogenate and the synaptosmal and neuronal perikarya fractions from 17-day-old rats were compared for their activities in sialosylating endogenous gangliosides and transferring N-acetylneuraminic acid and galactose to several glycolipids in vitro. The sialosylation of endogenous gangliosides and the activities of sialosyltransferases acting either on lactosylceramide or haematoside as acceptors, as well as galactosyltransferase acting on Tay-Sachs ganglioside as acceptor, were between 3-and 12-fold higher in the neuronal perikarya fraction than in whole homgenate on a protein or ganglioside basis. The activities found in the synaptosomal fraction were negligible. No evidence was found to indicate that the low activities in this fraction were due to the presence of inhibitors of the transfer activities or to inacessibility of the substrates to their respective enzymes. These findings, and the time course of labelling of gangliosides of the neuronal perikarya and synaptosomes from rats that received an injection of N-[3H]acetylmannosamine, indicate that the main cellular site of glycosylation of neuronal gangliosides is in the neuronal perikarya.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1185969 | PMC |
| http://dx.doi.org/10.1042/bj1740673 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Estrogen-regulated lateral septal kisspeptin neurons abundantly project to GnRH neurons and the hypothalamic supramammillary nucleus.
J Neurosci
January 2025
Laboratory of Reproductive Neurobiology, Hun-Ren Institute of Experimental Medicine, Budapest, 1083 Hungary;
While hypothalamic kisspeptin (KP) neurons play well-established roles in the estrogen-dependent regulation of reproduction, little is known about extrahypothalamic KP-producing (KP) neurons of the lateral septum. As established previously, expression in this region is low and regulated by estrogen receptor- and GABA receptor-dependent mechanisms. Our present experiments on knock-in mice revealed that transgene expression in the LS begins at P33-36 in females and P40-45 in males and is stimulated by estrogen receptor signaling.
View Article and Find Full Text PDFAging of Krushinsky-Molodkina audiogenic rats is accompanied with pronounced neurodegeneration and dysfunction of the glutamatergic system in the hippocampus.
Brain Res
January 2025
Sechenov Institute of Evolutionary Physiology and Biochemistry, the Russian Academy of Sciences, St. Petersburg, Russian Federation. Electronic address:
Advancing age strongly correlates with an increased risk of epilepsy development. On the other hand, epilepsy may exacerbate the negative effects of aging making it pathological. In turn, the possible link between aging and epileptogenesis is dysregulation of glutamatergic transmission.
View Article and Find Full Text PDFComplex Brain Morphology Discovered in the Shark Parasite Nybelinia surmenicola (Cestoda: Trypanorhyncha).
J Morphol
November 2024
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Article Synopsis
- - The study examines the complex structure of the nervous system in Nybelinia surmenicola, a type of cestode found in shark intestines, highlighting its unique brain architecture with nine distinct lobes and a protective extracellular capsule.
- - About 120 neurons are identified in the anterior lobes, with various types showing differences in size, shape, and synaptic structures, and two types of glial cells supporting neuronal function.
- - The findings suggest the brain's organization relates to the evolution and behavior of cestodes, indicating that the nervous system's anatomy plays a crucial role in their morphological and functional development.
Ultrastructural Analysis of the Large Neuronal Perikarya in an Injured Dentate Nucleus Using an Experimental Model of Hyperthermia-Induced Convulsions: The First Qualitative and Quantitative Study.
J Clin Med
September 2024
Department of Medical Pathomorphology, Faculty of Medicine with the Division of Dentistry and Division of Medical Education in English, Medical University of Bialystok, 15-269 Białystok, Poland.
Article Synopsis
- Febrile seizures are a common type of convulsion in kids, and this study aims to understand how they happen by looking closely at brain cells in rats.
- The researchers heated the rats to make them have seizures, then used special microscopes to see what happened to the brain cells.
- They found that the brain cells showed serious damage when the rats were heated, which could help scientists find ways to prevent seizures in children in the future.
Applying the area fraction fractionator (AFF) probe for total volume estimations of somatic, dendritic and axonal domains of the nigrostriatal dopaminergic system in a murine model.
J Neurosci Methods
October 2024
Laboratorio de Neuroanatomía, Departamento de Anatomía, and Centro Interdisciplinario de Neurociencia, NeuroUC, Escuela de Medicina, Pontificia Universidad Católica de Chile, Chile; Departamento de Neurociencia, Facultad de Medicina, Universidad de Chile, Chile. Electronic address:
Background: The Cavalieri estimator is used for volume measurement of brain and brain regions. Derived from this estimator is the Area Fraction Fractionator (AFF), used for efficient area and number estimations of small 2D elements, such as axons in cross-sectioned nerves. However, to our knowledge, the AFF has not been combined with serial sectioning analysis to measure the volume of small-size nervous structures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!