A significant loss in nucleolar volume density (proportion of nuclear volume occupied by nucleoli) occurs in the macronucleus as clonal age increases, which suggests an age-correlated loss of ribosomal RNA synthesizing capacity. In the macronuclear fragments of cells undergoing autogamy, however, a significant gain in nucleolar volume density takes place.

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http://dx.doi.org/10.1016/0047-6374(81)90101-9DOI Listing

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