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Örjan Ouchterlony and the antigen-antibody double diffusion-in-gel: a survey.
APMIS
January 2025
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
The Swedish scientist Örjan Ouchterlony published four ground-breaking papers 1948-1966 in Acta Pathol Microbiol Scand where he described a new method of antigen-antibody reactions in gel. He described and defined the 'reaction of identity' and 'reaction of partial identity' when he used related antigens and 'reaction of non-identity' when he used non-related antigens. His results inspired scientists in other countries to further develop and modify the 'Ouchterlony method' which became useful for both scientific and clinical purposes.
View Article and Find Full Text PDFA Laboratory Exercise Simulating Antibody and Antigen Reactions of the Ouchterlony Double Immunodiffusion Assay Using Inorganic Salts.
J Microbiol Biol Educ
June 2021
Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA.
The Ouchterlony double immunodiffusion assay is a serological technique used in the detection of antibodies and antigens for diagnostic purposes and also used in immunology laboratory courses as a common teaching assay where students observe the geometrical precipitation line patterns that form in the agarose, elucidating degrees of homology between antigens. In this classical technique, students must wait several hours to days to obtain results when protein antigens and antibodies are used. Furthermore, these proteins degrade over time if not frozen or stored in the refrigerator and are the most expensive consumables of the laboratory exercise.
View Article and Find Full Text PDFImmunoelectrophoresis: A Method with Many Faces.
Methods Mol Biol
June 2019
Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Immunoelectrophoresis (IEP) was the first practical method that combined electrophoresis and immunoprecipitation for identifying and characterizing proteins within complex mixtures. Over the years, IEP has been extended to include a variety of techniques and, as a general name, has been applied to virtually any technique that involves electrophoresis and antigen-antibody precipitin reaction for proteins. Because of the diversity in technical details of different IEP versions, the method described here deals only with classic IEP.
View Article and Find Full Text PDFImmunoelectrophoresis: a method with many faces.
Methods Mol Biol
September 2012
Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Immunoelectrophoresis (IEP) was the first practical method that combined electrophoresis and -immunoprecipitation for identifying and characterizing proteins within complex mixtures. Over the years, IEP has been extended to include a variety of techniques and, as a general name, has been applied to virtually any technique that involves electrophoresis and antigen-antibody precipitin reaction for proteins. Because of the diversity in technical details of different IEP versions, the method described here deals only with classic IEP.
View Article and Find Full Text PDFEnhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice.
Endocrinology
August 2010
Department of Medical Gene Technology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Graves-like hyperthyroidism is induced in BALB/c mice by immunization with adenovirus expressing the human TSH receptor (TSHR) A-subunit (amino acids 1-289). However, because of nonidentity between the human and mouse TSHR ( approximately 87% amino acid homology), we compared the responses of mice immunized with adenoviruses expressing either the mouse or the human TSHR A-subunit. Wild-type (wt) BALB/c mice immunized with the mouse A-subunit developed neither TSHR antibodies (measured by flow cytometry) nor thyroid lymphocytic infiltration.
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