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In vivo anthelmintic activity of hydroethanolic extract of Piper cubeba fruits in sheep naturally infected with gastrointestinal nematodes.

Vet Parasitol

January 2025

Universidade Estadual Paulista (Unesp), FCAT/Unesp - Faculdade de Ciências Agrárias e Tecnológicas Campus de Dracena, Rod. Cmte João Ribeiro de Barros, km 651 - Bairro das Antas, Dracena, São Paulo CEP 17900-000, Brazil. Electronic address:

Parasitic infections caused by gastrointestinal nematodes (GINs) are the main cause of production losses in small ruminants, especially sheep. Haemonchus contortus is the most common nematode in tropical regions. The indiscriminate use of synthetic anthelmintics to control helminthosis has led to the development of resistant parasites.

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Background: Parasitic infection remains a serious health trade for humans and livestock. The purpose of this study was to present scientific proof of the anthelmintic properties of , which the native population uses to cure helminthiasis.

Method: Fresh eggs were isolated from faecal samples of experimentally infected mice.

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Article Synopsis
  • Anthelmintic resistance in livestock is increasing globally, prompting researchers to explore alternative treatments, such as medicinal plants.
  • This study evaluates the anthelmintic effectiveness of crude ethanolic extract (CEE) from Juglans regia leaves against the parasitic nematode Ascaridia galli in poultry using both in vitro and in vivo experiments.
  • Results indicate that CEE significantly inhibits the motility of A. galli worms and shows promising anthelmintic activity in treated chickens, suggesting its potential as a natural alternative to synthetic drugs.
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Aim Of The Study: The growing resistance of helminth parasites to currently available commercial anthelmintic drugs, combined with apprehensions regarding detrimental chemical residues in livestock products, has sparked an interest in exploring medicinal plants as an alternative strategy for treating helminthiasis. As a result, this study was designed to investigate the anthelmintic activity of crude methanolic extracts (CME) of Saussurea costus root on Ascaridia.galli, a pathogenic nematode of poultry.

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() and () are widely found in flora of the Cholistan Desert of Bahawalpur, Pakistan and are traditionally used to treat pain and chronic disease. The current study aimed to evaluate their phytochemical screening, antioxidant activity, phagocytic activity, and analgesic activity. Both the plant extracts were investigated for phytochemical screening, Fourier Transform Infrared (FTIR) analysis, antioxidant by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, immunomodulatory activity by macrophages phagocytosis using carbon clearance rate assay and analgesic activity by acetic acid produced writhing method.

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