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Virus inactivation in hemoglobin solution by heat treatment.
Artif Cells Blood Substit Immobil Biotechnol
September 2001
Hokkaido Red Cross Blood Center, Sapporo, Japan.
To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60 degrees C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.
View Article and Find Full Text PDFChemical characterization of pyridoxalated hemoglobin polyoxyethylene conjugate.
Biochim Biophys Acta
January 2000
Apex Bioscience Inc., P.O. Box 12847, Research Triangle Park, NC 27709, USA.
Pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) was developed in the 1980s as an oxygen carrier and is now under development for treatment of nitric oxide-dependent, volume refractory shock. PHP is made by derivatizing human stroma-free hemoglobin with pyridoxal-5-phosphate and polyoxyethylene (POE). A unique aspect of using POE for modification is that unlike its mono-methoxy polyethylene glycol (PEG) relatives, POE is bifunctional.
View Article and Find Full Text PDFBis[2-(4-carboxyphenoxy)carbonylethyl]phosphinic acid (BCCEP): a new reagent for hemoglobin modification.
Artif Cells Blood Substit Immobil Biotechnol
November 1997
Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21250, USA.
The synthesis and hemoglobin cross-linking studies of a novel organic reagent, bis[2-(4-carboxyphenoxy)carbonylethyl]phosphinic acid (BCCEP; 2) has been reported. The reagent was synthesized in four steps from hydroxybenzoic acid. The tri-sodium salt of BCCEP was employed to cross-link oxyHb, and the product was purified by DEAE-cellulose chromatography.
View Article and Find Full Text PDFCharacteristics of Hb-vesicles and encapsulation procedure.
Artif Cells Blood Substit Immobil Biotechnol
January 1995
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca.
View Article and Find Full Text PDFApplication of aqueous two-phase systems in separation/purification of stroma free hemoglobin from animal blood.
Artif Cells Blood Substit Immobil Biotechnol
January 1995
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Aqueous two-phase system (ATPS) is suitable for treatment of biological materials. It has been traditionally employed in the separation of product from fermentation broth, and in the extraction of large-molecule proteins from colloid solution. The separation method based on ATPS features simple, rapid and continuous operation with low equipment cost.
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