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HILIC-MS/MS Multi-targeted Method for Metabolomics Applications.
Methods Mol Biol
January 2025
Biomic Auth, Bioanalysis and Omics Laboratory, Center for Interdisciplinary Research and Innovation, Aristotle University, Thessaloniki, Greece.
Metabolomics aims at identification and quantitation of key end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays enjoying a growing popularity over the last years with LC-MS/MS as a powerful tool for development of such (semi-)quantitative methods for a large number of metabolites. Here we describe a method for absolute quantitation of ca.
View Article and Find Full Text PDFCarotenoid profile in maternal and cord plasma and its trends in breast milk during lactation: a comparative study among three cities in northern China.
Food Funct
January 2025
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Increasing evidence suggests that carotenoids play an important role in visual and cognitive development during early life. This study aimed to depict the carotenoid profile in maternal/cord plasma and breast milk in three northern cities of China while investigating the association between dietary carotenoid intake and breast milk carotenoid levels. We enrolled 990 lactating mothers from three urban northern Chinese cities to collect breast milk (including colostrum, transitional milk, early mature milk, middle mature milk, and late mature milk).
View Article and Find Full Text PDFScreening and characterizing indigenous yeasts, lactic acid bacteria, and acetic acid bacteria from cocoa fermentation in Hainan for aroma Development.
J Food Sci
January 2025
Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, China.
Fermentation is crucial for inducing desirable flavor and aroma profiles in cocoa products. This research focused on identifying microbial strains isolated from spontaneous cocoa fermentation in Hainan through 16S and Internal Transcribed Spacer (ITS) sequencing. Pectinase activity was screened, and metabolic dynamics of sugars and organic acids were analyzed using high-performance liquid chromatography.
View Article and Find Full Text PDFG-Quadruplex DNAzyme-Based Biocatalysis Combined with an Intelligent Electromagnetic-Actuated Microfluidic Chip for Tetracycline Detection.
J Agric Food Chem
January 2025
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
In this study, we present an intelligent electromagnetic-actuated microfluidic chip integrated with a G-quadruplex DNAzyme-based biocatalysis platform for rapid and sensitive tetracycline (TC) detection. In this sensing system, TC significantly quenches fluorescent magnetic carbon dots (M-CDs) via the internal filtration effect and dynamic quenching (the excitation and emission wavelength at 350 and 440 nm, respectively). Then, the G-quadruplex on the M-CDs-Aptamer is exposed and bound with hemin to form hemin-G-quadruplex DNAzyme, catalyzing the conversion of 3,3',5,5'-tetramethylbenzidine to produce blue color.
View Article and Find Full Text PDFCustomizable large-scale HPLC fraction collection using low-cost 3D printing.
HardwareX
March 2025
Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA.
High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current commercially available fraction collectors are not easily customizable, incompatible with other platforms, and come with a large cost barrier making them inaccessible to many researchers.
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