Investigations were carried out with homogenates from the organs of hens (liver, duodenum, pancreas, spleen, kidney, glandular and muscular stomach, ovary resp. testicle, periosteum,) as well as serums in which the general activity of the alkaline phosphatase was determined and parallel to this after an inhibition in the presence of 10 mM 1-phenylalanine or different concentrations (0.25--4 M) or urea, as well as with values of pH ranging from 9.0 up to 11.0, after a preincubation of urea for 10, 20 and 30 min with 3 M/l of after heating up to 56 degrees C for 10, 20 and 30 min. An electrophoresis of an agar gel was also carried out. Some stereospecific peculiarities of the alkaline phosphatase isoenzymes of the organs studied was found with regard to the inhibitors used. The selective inhibition, as well as the electrophoretic division of enzymes, did not produce any considerable differences, which can be used for practical ends.
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