A description is given of a gas-liquid chromatographic method for the quantitative determination of unchanged prenalterol in plasma and for the total (free and conjugated) prenalterol in urine. After addition of an adequate internal standard, prenalterol together with the internal standard, is extracted with diethyl ether and derivatized with heptafluorobutyric anhydride-pyridine to form a tri-heptafluorobutyric derivative. This derivative has favourable properties for its estimation by gas-liquid chromatography using electron-capture detection. A large percentage of prenalterol is excreted as sulfate conjugate in man. Thus a hydrolysis step is added to the urine assay. The sensitivity of the method is about 2ng/ml.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0378-4347(00)84144-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adrenoceptor subtypes in the control of burn-induced plasma extravasation.
Burns
March 2005
Department of Anaesthesia and Intensive Care and Institution of Surgical Specialties, Sahlgrenska University Hospital, Mölndal, Sweden.
Burn trauma is known to induce a significant rise in circulating catecholamine levels and despite catecholamines being potent endogenous vasoactive agents with known actions on microvascular permeability, their effect on burn edema has been poorly investigated. The present study in rats investigated the role and importance of adrenergic receptor subtypes in the regulation of basal capillary permeability in normal skin and hyperpermeability in partial- and full-thickness skin burns. Edema was quantified by spectrophotometric analysis of extravasated Evans blue-albumin.
View Article and Find Full Text PDFEffects of sympathetic activation on plasma renin activity in the developing rat.
J Pharmacol Exp Ther
April 1990
Department of Psychology, University of Iowa, Iowa City.
The present experiments studied the ontogeny of sympathetic control of the renin-angiotensin system by using pharmacological agents, which act at different levels of the sympathetic axis, to increase plasma renin activity (PRA) during the preweanling period in Sprague-Dawley rats. The selective beta-1 adrenoceptor agonist, prenalterol, produced age- and dose-dependent PRA increases. In 5- and 10-day-old animals, prenalterol treatment produced minimal stimulation of PRA and the dose-response curve was essentially flat.
View Article and Find Full Text PDFA strategy for the determination of beta blockers in plasma using solid-phase extraction in combination with high-performance liquid chromatography.
J Pharm Biomed Anal
August 1991
Vrije Universiteit Brussel, Farmaceutisch Instituut, Belgium.
This paper describes a general approach for the therapeutic drug monitoring of 13 different beta blockers in plasma. The chromatographic system contains a cyanopropyl-bonded phase as a stationary phase in combination with a mobile phase composed of acetonitrile and phosphate buffer (pH = 3, mu = 0.05).
View Article and Find Full Text PDFEffects of prenalterol on renal function in patients after major vascular surgery.
Crit Care Med
May 1988
Department of Anesthesiology, Karolinska Hospital, Stockholm, Sweden.
Hemodynamic and renal effects of prenalterol were studied in 13 mechanically ventilated patients on the first day after major vascular surgery. Prenalterol (1-[4-hydroxyphenoxy]-3-isopropylamino-2 propanol hydro-chloride), a partial beta-agonist with a predominant beta-1 and a weak beta-2-adrenoceptor activity, was infused into seven patients at rates of 0.5 and 1.
View Article and Find Full Text PDFMetabolic and hormonal effects of preferential beta 1 and beta 2-adrenoceptor stimulation in man.
J Endocrinol Invest
April 1988
CNR Respiratory Unit, University of Perugia, Italy.
The changes of blood glucose, serum potassium (K+), plasma non-esterified fatty acids (NEFA), plasma insulin and plasma renin activity (PRA) following the preferential stimulation of beta-1 and beta-2 adrenoceptors were studied in 7 healthy subjects during a 60-min infusion of prenalterol, a new, relatively beta-1 selective agonist and salbutamol, a well known, relatively beta-2 selective agonist. Two different high and low doses were used for both agents: 300 micrograms and 600 micrograms and 1 mg and 2 mg for salbutamol and prenalterol, respectively. The levels of PRA seemed equally increased by the two agents in proportion to the dose infused, thus suggesting an equally important role for beta-1 and beta-2 adrenoceptors in renin release.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!